Search results for "Starch gel electrophoresis"

showing 2 items of 2 documents

Two tissue-specific loci for octopine dehydrogenase in Tapes Decussatus (Bivalvia, Veneridae)

1992

Abstract 1. 1. Octopine dehydrogenase catalyses the reductive condensation of pyruvate and arginine to produce octopine in mollusc tissues. 2. 2. In all Bivalvia species studied to date, a single polymorphic locus, anodally migrating, was demonstrated. 3. 3. We studied, by starch gel electrophoresis, octopine isozymes in Tapes decussatus tissues. 4. 4. A polymorphic anodal form is present in adductor muscle, foot muscle, mantle and gill. 5. 5. A hepatopancreas-specific form, cathodally migrating, is also evident.

OctopinebiologyPhysiologyD-octopine dehydrogenaseDehydrogenaseVeneridaeGeneral MedicineBivalviabiology.organism_classificationBiochemistryIsozymechemistry.chemical_compoundStarch gel electrophoresisBiochemistrychemistryBotanyMantle (mollusc)Molecular BiologyComparative Biochemistry and Physiology Part B: Comparative Biochemistry
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Methods for Separating Native Enzymes

1994

In the course of electrophoresis the stability of an enzyme depends on such conditions as (a) pH-value, (b) ion strength and ion species, (c) effector molecules, (d) temperature and (e) properties of the separation matrix. These parameters were empirically optimized for starch gel electrophoresis [1–3] and cellulose acetate electrophoresis [4, 5] when analyzing predominantly animal and human specimen. A major advantage of these types of separation media is that practically every buffer system can be used to separate enzymes whereas in disc-gel electrophoresis [6–8] the number of applicable buffer systems is limited. When using isoelectric focusing to separate native enzymes no buffer choice…

chemistry.chemical_classificationGel electrophoresisElectrophoresisStarch gel electrophoresisIsoelectric pointChromatographyEnzymeMolecular massChemistryIsoelectric focusingBuffer (optical fiber)
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